Effect of cell culture on 18S rRNA gene sequences in the cultural course of Taxus chinensis cells.
نویسندگان
چکیده
Cell culture is an effective technology for taxol production. This paper discusses the effect of Taxus cell cultures on the 18S rRNA gene sequences based on the phylogenetic analysis of cultured T. chinensis cells and related species. The phylogenetic tree is reconstructed using the maximum parsimony method and the relative rate test to test the hypothesis of a molecular clock. The phylogenetic analysis indicates that cell culture changes the phylogenetic position of cultured T. chinensis cells. More than that, the 18S rRNA gene of cultured T chinensis cells has a faster rate of substitution than that of T. chinensis. With T. media as reference, the divergence time of the cultured T. chinensis cells is 7 Ma (million years) more than that of the T. chinensis cells based on the 18S rRNA gene sequences.
منابع مشابه
Effect of DNA methylation on 18S rRNA gene sequences during culture of Taxus chinensis cells.
* Author for correspondence and reprint requests Z. Naturforsch. 64c, 418-420 (2009); received December 15, 2008 Cell suspension culture has rapidly become an alternative source of taxol, an anticancer compound. To investigate the role of DNA methylation in the cultural course of Taxus chinensis cells, analyses of 18S rRNA gene sequences of cultured T chinensis cells and related species were co...
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ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. C, Journal of biosciences
دوره 63 1-2 شماره
صفحات -
تاریخ انتشار 2008